Journal: Journal of Orthopaedic Translation

Article Title: Charting a quarter-century of commercial cartilage regeneration products

doi: 10.1016/j.jot.2024.10.009

Figure Lengend Snippet: Information on the 39 commercial products.

Article Snippet: JAAC® , 2012, MHLW-PMDA , Japan Tissue Engineering Co., Ltd. , Atelocollagen solution (3 % Type 1 collagen) , https://www.jpte.co.jp/en/business/regenerative/cultured-cartilage/index.html.

Techniques: Membrane, Cell Culture, Clinical Proteomics, Produced

Journal: Frontiers in Immunology

Article Title: Col1A1 as a new decoder of clinical features and immune microenvironment in ovarian cancer

doi: 10.3389/fimmu.2024.1496090

Figure Lengend Snippet: COL1A1 mRNA expression levels in cancer. (A) Utilizing the Gent2 database, we conducted a comparative analysis of COL1A1 expression levels in various pan-cancerous tumors and their adjacent normal tissues. (B) A examination of COL1A1 expression in 22 human cancer types was performed using the TNM plot database. The results indicated significant differences in COL1A1 expression between cancer and normal tissues, with these differences being marked with red asterisks (*) to highlight their statistical significance. (C) By integrating data from Human Protein Atlas (HPA), we investigated the mRNA expression profile of COL1A1 in 17 human cancer types. The combined analysis showed that COL1A1 expression showed relatively low specificity in cancer, suggesting that its expression may not be restricted to specific cancer types. The expression of COL1A1 in the tissues of OC is indicated by red arrows.

Article Snippet: The sections were then cooled to room temperature (RT) and incubated with COL1A1 primary antibody solution (Proteintech, 67288-1-Ig) for 1 hour at RT.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: Col1A1 as a new decoder of clinical features and immune microenvironment in ovarian cancer

doi: 10.3389/fimmu.2024.1496090

Figure Lengend Snippet: Analysis of COL1A1 expression in OC. (A) Single-cell sequencing data demonstrated that COL1A1 expression was dominantly observed in connective tissue cells. Cluster 50 was identified as the primary source of COL1A1 expression, as indicated by the red arrow. (B) The typical location of COL1A1 protein was in endoplasmic reticulum. Target protein was marked by green fluorescence. (C) Summarization of COL1A1 expression in 59 OC cell lines (nTPM ≥ 0.1). (D) Protein levels of COL1A1 were evaluated in normal ovarian tissue using immunohistochemical methods. (E) Protein levels of COL1A1 were evaluated in OC tissues using immunohistochemical methods.

Article Snippet: The sections were then cooled to room temperature (RT) and incubated with COL1A1 primary antibody solution (Proteintech, 67288-1-Ig) for 1 hour at RT.

Techniques: Expressing, Sequencing, Fluorescence, Immunohistochemical staining

Journal: Frontiers in Immunology

Article Title: Col1A1 as a new decoder of clinical features and immune microenvironment in ovarian cancer

doi: 10.3389/fimmu.2024.1496090

Figure Lengend Snippet: High expression of COL1A1 is associated with the clinicopathological features of OC patients. (A, B) Analysis of CHIP data (A) and RNA-Seq data (B) from the TNMplot database revealed that COL1A1 expression in OC tissue samples was significantly elevated compared to that in normal ovarian tissue (P<0.05). (C) Analysis of COL1A1 mRNA expression in the TNMplot database demonstrated a gradual increase in COL1A1 expression from normal tissues to tumor tissues and then to metastatic tissues in OC patients. (D) The relationship between the pathological stage of OC and COL1A1 expression. (E, F) Overall survival (E) and relapse-free survival (F) curves were generated for OC patients with different expression levels of COL1A1 using data from the KM plotter database. (G-I) Representative images (G) of COL1A1 IHC staining were presented, alongside statistical results of COL1A1 IHC scores in normal ovarian tissue samples (n=11) (H) , and lymph node metastasis-negative (LNM - ) and lymph node metastasis-positive (LNM + ) OC tissue samples (I) . Significant differences in COL1A1 IHC scores were observed between normal and OC tissues, as well as between LNM- and LNM+ OC tissues (normal ovarian tissue samples, n=11, LNM - tissue samples, n=11, and LNM + tissue samples, n=8. **p<0.01).

Article Snippet: The sections were then cooled to room temperature (RT) and incubated with COL1A1 primary antibody solution (Proteintech, 67288-1-Ig) for 1 hour at RT.

Techniques: Expressing, RNA Sequencing, Generated, Immunohistochemistry

Journal: Frontiers in Immunology

Article Title: Col1A1 as a new decoder of clinical features and immune microenvironment in ovarian cancer

doi: 10.3389/fimmu.2024.1496090

Figure Lengend Snippet: Correlation Between COL1A1 mRNA Expression Levels and OS and PFS in OC Patients with Different Clinicopathological Characteristics.

Article Snippet: The sections were then cooled to room temperature (RT) and incubated with COL1A1 primary antibody solution (Proteintech, 67288-1-Ig) for 1 hour at RT.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: Col1A1 as a new decoder of clinical features and immune microenvironment in ovarian cancer

doi: 10.3389/fimmu.2024.1496090

Figure Lengend Snippet: Correlation between COL1A1 expression and immune infiltration in OC according to the TIMER database. (A-C) COL1A1 expression is tightly correlated with the recruitment of B cells (A) , CD4 + T cells (B) , and CD8 + T cells (C) in OC. (D-F) The correlation between the COL1A1 expression and the recruitments of Macrophages (D) , Neutrophils (E) and Dendritic cells (F) in OC tissues. (G-I) Scatter plots of COL1A1 expression in OC correlating withPD-1 (G) , PD-L1 (H) , and CTLA-4 (I) using the GEPIA database.

Article Snippet: The sections were then cooled to room temperature (RT) and incubated with COL1A1 primary antibody solution (Proteintech, 67288-1-Ig) for 1 hour at RT.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: Col1A1 as a new decoder of clinical features and immune microenvironment in ovarian cancer

doi: 10.3389/fimmu.2024.1496090

Figure Lengend Snippet: Survival curves of high and low expression of COL1A1 in OC in diverse immune cell subgroups. (A) B cells. (B) Macrophages. (C) CD8+ T cells. (D) CD4+ memory T cells. (E) Type 1 T-helper cells. (F) Type 2 T-helper cells. (G) Regulatory T cells. (H) Natural killer T cells. (I) Eosinophils. (J) Basophils. (K) Mesenchymal stem cells.

Article Snippet: The sections were then cooled to room temperature (RT) and incubated with COL1A1 primary antibody solution (Proteintech, 67288-1-Ig) for 1 hour at RT.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: Col1A1 as a new decoder of clinical features and immune microenvironment in ovarian cancer

doi: 10.3389/fimmu.2024.1496090

Figure Lengend Snippet: Association of COL1A1 expression level with immune cell infiltration. (A-E) Association between COL1A1 and abundance of TILs with four most significant molecules in the Tumor Immune System Interaction Database (TISIDB). (F-J) Correlations between chemokines and COL1A1 with four most significant molecules identified in the TISIDB. Red box indicates the changes in molecular abundance in OC.

Article Snippet: The sections were then cooled to room temperature (RT) and incubated with COL1A1 primary antibody solution (Proteintech, 67288-1-Ig) for 1 hour at RT.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: Col1A1 as a new decoder of clinical features and immune microenvironment in ovarian cancer

doi: 10.3389/fimmu.2024.1496090

Figure Lengend Snippet: Correlation between COL1A1 expression and immunomodulators. (A-E) The correlation of immunoinhibitors and four most important molecules with COL1A1 expression in the TISIDB. (F-J) The correlation of immunostimulators and four most important molecules with COL1A1 expression in the TISIDB. Red box indicates the changes in molecular abundance in OC.

Article Snippet: The sections were then cooled to room temperature (RT) and incubated with COL1A1 primary antibody solution (Proteintech, 67288-1-Ig) for 1 hour at RT.

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: Col1A1 as a new decoder of clinical features and immune microenvironment in ovarian cancer

doi: 10.3389/fimmu.2024.1496090

Figure Lengend Snippet: Gene associated with COL1A1 in OC. (A) Genes associated with COL1A1 in OC analyzed by Spearman Correlation test. (B) Positively correlated significant genes of COL1A1 in OC analyzed by Spearman Correlation test. (C) Negatively correlated significant genes of COL1A1 in OC analyzed by Spearman Correlation test. (D-I) Correlation analysis between the top six positively correlated differential genes and COL1A1, including COL5A1 (D) , COL6A3 (E) , AEBP1 (F) , COL1A2 (G) , SSC5D (H) , ITGA5 (I) .

Article Snippet: The sections were then cooled to room temperature (RT) and incubated with COL1A1 primary antibody solution (Proteintech, 67288-1-Ig) for 1 hour at RT.

Techniques:

Journal: Frontiers in Immunology

Article Title: Col1A1 as a new decoder of clinical features and immune microenvironment in ovarian cancer

doi: 10.3389/fimmu.2024.1496090

Figure Lengend Snippet: Enrichment results of the top 100 differential genes associated with COL1A1 in OC. (A) The KEGG functional enrichment analysis identified biological pathways and metabolic networks that are closely related to these molecules. (B-D) The GO annotation technique annotated the biological functions of these molecules, including biological process (BP), cellular component (CC), and molecular function (MF).

Article Snippet: The sections were then cooled to room temperature (RT) and incubated with COL1A1 primary antibody solution (Proteintech, 67288-1-Ig) for 1 hour at RT.

Techniques: Functional Assay